赵丽丽,朱清澄,花传祥.基于线粒体细胞色素 b 的秋刀鱼种群遗传结构的研究[J].海洋通报,2019,(3):
基于线粒体细胞色素 b 的秋刀鱼种群遗传结构的研究
Genetic structure of saury population based on mitochondrial cytochrome b sequence analysis
投稿时间:2018-08-23  修订日期:2018-11-27
DOI:10.11840/j.issn.1001-6392.2019.03.010
中文关键词:  秋刀鱼  细胞色素 b 基因  遗传结构  遗传多样性
英文关键词:Cololabis saira  cytochrome b gene  genetic structure  genetic diversity
基金项目:国家科技支撑计划 (2013BAD13B05)
作者单位E-mail
赵丽丽 上海海洋大学 海洋科学学院上海 201306 982825580@qq.com 
朱清澄 上海海洋大学 海洋科学学院上海 201306上海海洋大学 国家远洋渔业工程技术研究中心上海 201306上海海洋大学 大洋渔业资源可持续开发省部共建教育部重点实验室上海 201306 qczhu@shou.edu.cn 
花传祥 上海海洋大学 海洋科学学院上海 201306上海海洋大学 国家远洋渔业工程技术研究中心上海 201306上海海洋大学 大洋渔业资源可持续开发省部共建教育部重点实验室上海 201306  
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中文摘要:
      秋刀鱼主要分布在西北太平洋亚热带到温带水域中,是一种资源量较高的上层洄游性鱼类。为了对秋刀鱼的遗传结构进行研究,2016年 6月-11月,在 38?38"-44?31"N,146?36"-162?26"E 海域随机取 145 条秋刀鱼,取其肌肉与DNA、PCR以及测序后获得 421 bp 基于线粒体细胞色素 b (Cytb) 基因的 DNA 序列。分析结果显示,145 条序列中共发现了 15 个单倍型,单倍型多样性指数 h = 0.258± 0.049,核苷酸多样性π = 0.000 87±0.007 56。计算得出 Tajima’s D 与 Fu’s Fs 均呈负值并且绘制的核苷酸不配对曲线呈现单峰,可得出秋刀鱼群体经历了种群扩张。Nm 值表明 4 个采样点的秋刀鱼群体之间存在频繁的基因交流,AMOVA 分析结果显示 97.22 %的遗传变异来自群体内,结合遗传分化系数 Fst 得出群体遗传分化水平较低。单倍型网络图呈现星状拓扑结构、系统发育树分枝不明显均显示地域性差异不显著。研究发现秋刀鱼群体遗传多样性较低,需对其进行合理保护。
英文摘要:
      Saury, a high-capacity upper migratory fish, is mainly distributed in the subtropical to temperate waters of the Northwestern Pacific. From June 2016 to November 2016, 145 random samplings was performed in sea area at 38?38"-44?31"N, 146? 36" -162? 26" E, Taking DNA from the muscle, the mitochondrial were found in 145 sequences. with the haplotype diversity index h=0.258±0.049, and the nucleotide diversity index π = 0.000 87±0.007 56. It was calculated that both Tajima’s D and Fu’s Fs were negative and the nucleotide unpaired curve was a single peak, which indicated that the saury population experienced population expansion. Fst analysis of gene exchange and poor differentiation coefficient indicated that there were frequent gene exchanges between the saury populations at the four sampling points. The results of AMOVA analysis showed that 97.22 % of the genetic variation came from the population, and the genetic differentiation of the population was not significant. The haplotype network diagram presented a star -like topology, and the phylogenetic tree branches were not obvious, and the regional differences were not significant. In this study, it was found that the saury population had low genetic diversity and needed to be protected reasonably.
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