蔡晓芳,李庆昌,张晓林,历俊帆,刘贤德.大黄鱼皮质醇无创检测技术研究[J].海洋通报,2019,(5):569-573
大黄鱼皮质醇无创检测技术研究
Study on the non-invasive measuring technology of cortisol in large yellow croaker (Larimichthys crocea)
投稿时间:2018-06-22  修订日期:2018-08-30
DOI:10.11840/j.issn.1001-6392.2019.05.011
中文关键词:  大黄鱼  无创检测  皮质醇  高温胁迫
英文关键词:Larimichthys crocea  non-invasive measurement  cortisol  high temperature stress
基金项目:福建省自然科学基金(2019J01694);福建省高校新世纪人才支持计划(JA14167);福建省大学生创新训练项目(201810390051)
作者单位E-mail
蔡晓芳 集美大学水产学院农业农村部东海海水健康养殖重点实验室福建 厦门361021 2921611402@qq.com 
李庆昌 集美大学水产学院农业农村部东海海水健康养殖重点实验室福建 厦门361021  
张晓林 集美大学水产学院农业农村部东海海水健康养殖重点实验室福建 厦门361021  
历俊帆 集美大学水产学院农业农村部东海海水健康养殖重点实验室福建 厦门361021  
刘贤德 集美大学水产学院农业农村部东海海水健康养殖重点实验室福建 厦门361021 xdliu@jmu.edu.cn 
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中文摘要:
      皮质醇是衡量鱼类应激状况的重要参考指标,从血液中检测皮质醇的常规方法会对鱼造成严重伤害甚至致其死亡。本实验室开发了一种从水中检测皮质醇的技术,应用该技术比较了大黄鱼高温处理组(28℃) 和对照组(20 ℃) 血清与水样中皮质醇含量的变化,结果显示血清与水样皮质醇的检测结果具有高度的一致性,且相关性显著(P < 0.05),说明通过测定水样中的皮质醇来代替血清中的皮质醇是可行的。为进一步验证该方法的效果,我们又比较了机械应激前后水样中皮质醇含量变化,结果显示机械胁迫也导致了皮质醇含量的显著升高(P < 0.01)。本技术的建立可为大黄鱼养殖过程中应激状态的无创检测提供参考。
英文摘要:
      Cortisol is an important reference indicator to measure the stress status of fish. Conventional detection method of cortisol need sampled blood from fish, which may cause fish injury or even death. In this study, a new technology is developed which can detect cortisol concentration in water. Using this technology the cortisol content in serum and water samples under the condition of high-temperature stress (28℃) and normal temperature (20℃) in large yellow croaker are firstly compared. The results showed that the trend of cortisol in serum and water samples were highly consistent and correlated significantly (P<0.05), which suggested the feasibility of this method to measure cortisol in water and can replace cortisol detection in serum. To further verify the effectiveness of the method, the cortisol content in water samples before and after mechanical stress are compared. The results showed that mechanical stress also led to a significant increase of cortisol content in water samples (P<0.01). The technology developed in this experiment can provide reference for non-invasive monitoring of stress state during the culture of large yellow croaker.
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